Sustained-release formulation of levodopa methyl ester/benserazide for prolonged suppressing dyskinesia expression in 6-OHDA-leisoned rats

► LDME/benserazide-loaded biodegradable microspheres decreased apomorphine-induced rotations in PD rats. ► Decreased dyskinesia expression in dyskinesia rats. ► Improved motor performance and prevented the increases in ΔFosB expression. ► Improved ERK1/2 activation and DARPP-32 phosphorylation in levodopa-treated rats.