Peroxynitrite treatment reduces adenosine uptake via the equilibrative nucleoside transporter in rat astrocytes

► We examined effect of oxidative stress on adenosine clearance by astrocytes. ► Oxidative stress with SIN-1 decreased the adenosine uptake. ► There was no alteration in expression of a responsible transporter. ► The affinity of adenosine to the transporter was decreased. ► Decreased adenosine clearance contributes to elevation of its extracellular level.