کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6285844 | 1296890 | 2008 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
αII-Spectrin breakdown product increases in principal cells in the gerbil main olfactory bulb following transient ischemia
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
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چکیده انگلیسی
In pathological states, calpains in neurons easily degrade spectrin and yield specific fragments called spectrin breakdown products characterized by their high stabilities both in vitro and in vivo. In the present study, we investigated chronological changes in αII-spectrin immunoreactivity and protein levels using the antibody to detect both the naïve form and breakdown products of αII-spectrin in the gerbil main olfactory bulb (MOB) after 5 min of transient forebrain ischemia. In sham-operated gerbils, weak αII-spectrin immunoreactivity was detected in principal (mitral and tufted) cells. Ten days after ischemia/reperfusion, αII-spectrin immunoreactivity was increased in principal cells. Fifteen days after ischemia/reperfusion, αII-spectrin immunoreactivity in the somata and processes of principal cells was markedly increased. Thereafter, αII-spectrin immunoreactivity in the principal cells in the ischemic MOB was decreased with time. In Western blot study, spectrin protein bands were detected in naïve form (230 kDa) and its breakdown product (150 kDa). The breakdown product in MOB homogenates were significantly increased 15 days after ischemia/reperfusion and thereafter decreased with time after ischemia/reperfusion. Our results indicate that αII-spectrin breakdown product in the gerbil MOB is changed in principal cells after ischemic insult.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience Letters - Volume 435, Issue 3, 25 April 2008, Pages 251-256
Journal: Neuroscience Letters - Volume 435, Issue 3, 25 April 2008, Pages 251-256
نویسندگان
In Koo Hwang, Ki-Yeon Yoo, Dae Won Kim, Hua Li, Ok Kyu Park, Choong Hyun Lee, Jung Hoon Choi, Moo-Ho Won,