Purification, characterization and decolorization of bilirubin oxidase from Myrothecium verrucaria 3.2190

► The bilirubin oxidase (BOD) from Myrothecium verrucaria 3.2190 was purified and characterized. ► Several enzymatic properties of bilirubin oxidase were investigated. ► The purified bilirubin oxidase was confirmed by MALDI-TOF-MS. ► Both Myrothecium verrucaria and the purified bilirubin oxidase can decolorize indigo carmine.