کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6288830 1301528 2013 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Monitoring the succession of the biota grown on a selective medium for pseudomonads during storage of minced beef with molecular-based methods
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
پیش نمایش صفحه اول مقاله
Monitoring the succession of the biota grown on a selective medium for pseudomonads during storage of minced beef with molecular-based methods
چکیده انگلیسی

In the present study, the succession of the biota grown on a selective medium for pseudomonads (pseudomonas agar based medium - PAB) during the storage of meat under different conditions was monitored. Thus, minced beef was stored aerobically and under modified atmosphere packaging in the presence (MAP+) and absence (MAP−) of oregano essential oil at 0, 5, 10 and 15 °C. A total of 267 pure cultures were recovered from PAB throughout the storage period and subjected to PCR-Denaturing gradient gel electrophoresis (PCR-DGGE) for their differentiation. In parallel, the direct analysis of the whole cultivable community (WCC) from the same medium was applied. These two approaches were used in order to indicate the lack of selectivity. Fifteen different DGGE fingerprints were obtained after PCR - DGGE analysis of the isolates, which were assigned to Pseudomonas putida (3 fingerprints), Pseudomonas fragi and Pseudomonas fluorescens, Pseudomonas spp., Serratia liquefaciens (2), Citrobacter freundii, Serratia grimesii, Hafnia alvei (3), Rahnella spp. and Morganella morganii. Twelve of them occurred during the direct analysis of the WCC. The biota succession found to be affected from the different storage conditions. However, the outcome of the two strategies was quite different, which is leading to the use of different appropriated molecular approaches in order to widen the knowledge of bacterial succession of meat.

► Succession of the biota of a selective growth medium for pseudomonads was studied. ► PCR-denaturing gradient gel electrophoresis was applied. ► Biota succession found to be affected from the different storage conditions. ► Pseudomonas fragi was detected in the most conditions adopted. ► Pseudomonas putida was found unable to grow under elevated temperatures.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Microbiology - Volume 34, Issue 1, May 2013, Pages 62-69
نویسندگان
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