کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6289372 | 1616330 | 2013 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Biodegradation of benzoate by protoplast fusant via intergeneric protoplast fusion between Pseudomonas putida and Bacillus subtili
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
علوم زیست محیطی (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Biodegradation of benzoate by protoplast fusant via intergeneric protoplast fusion between Pseudomonas putida and Bacillus subtili Biodegradation of benzoate by protoplast fusant via intergeneric protoplast fusion between Pseudomonas putida and Bacillus subtili](/preview/png/6289372.png)
چکیده انگلیسی
Intergeneric protoplast fusion was performed between Pseudomonas putida, a strain with moderate benzoate tolerance and degradation ability (<2000 mg lâ1) while slow growth rate (specific growth rate μ = 4.5 Ã 10â3 hâ1) and Bacillus subtilis, a strain with fast growth rate while intolerance to benzoate. A stable fusant (F29) with enhanced benzoate tolerance (as high as 10 000 mg lâ1) and degradation ability (μ = 30.9 Ã 10â3 hâ1) was obtained. The fusant is similar to P. putida in terms of amplified 16S ribosomal DNA restriction analysis (16S-ARDRA) and 16S rDNA clone sequencing analysis while alike to B. subtilis according to Gram staining. Possible biodegradation pathway of benzoate by F29 is benzene ring cleavage between two hydroxyl groups (ortho cleavage) with catechol being intermediate metabolite. However, the verification of benzoate biodegradation mechanism by this fusant requires further investigation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Biodeterioration & Biodegradation - Volume 85, November 2013, Pages 577-582
Journal: International Biodeterioration & Biodegradation - Volume 85, November 2013, Pages 577-582
نویسندگان
Mi Li, Peng Yi, Qiang Liu, Yun Pan, Guangren Qian,