Effect of DNA polymerases on PCR-DGGE patterns

► Influence of three DNA polymerases in PCR-DGGE, and their humic acid resistance was compared. ► Differences were observed between the produced fingerprints, especially in the number of detectable GC-rich amplicons. ► KOD DNA polymerase exhibited more bands than Taq, and showed the highest resistance against humic acids. ► KOD DNA polymerase seems beneficial for the analysis of challenging DNA templates.