Inactivation behavior of Pseudomonas aeruginosa by supercritical N2O compared to supercritical CO2

► SC N2O treatment shows strong bactericidal effect and releases a higher amount of intracellular substances from P. aeruginosa cells compared to SC CO2 treatment. ► SC N2O treatment affects relatively mild damage of enzyme activity, protein, and cell morphology compared to SC CO2 treatment. ► Mixing intensity is found to be the most important affecting parameter on SC N2O treatment.