Full length articleCymbopogon citratus-synthesized gold nanoparticles boost the predation efficiency of copepod Mesocyclops aspericornis against malaria and dengue mosquitoes

- Biosynthesized gold nanoparticles (AuN) were studied by FTIR, TEM, EDX and XRD.
- LC50 of AuN vs. Anopheles stephensi and Aedes aegypti ranged from 18.8 to 41.5 ppm.
- Copepod predation vs. A. stephensi and A. aegypti young larvae were 26.8% and 56%.
- In AuN-treated sites, predation was 45.6% (A. stephensi ) and 77.3% (A. aegypti).
- Low doses of AuN may boost the control of mosquito larvae in biocontrol programs.

Plant-borne compounds can be employed to synthesize mosquitocidal nanoparticles that are effective at low doses. However, how they affect the activity of mosquito predators in the aquatic environment is unknown. In this study, we synthesized gold nanoparticles (AuN) using the leaf extract of Cymbopogon citratus, which acted as a reducing and capping agent. AuN were characterized by a variety of biophysical methods and sorted for size in order to confirm structural integrity. C. citratus extract and biosynthesized AuN were tested against larvae and pupae of the malaria vector Anopheles stephensi and the dengue vector Aedes aegypti. LC50 of C. citratus extract ranged from 219.32 ppm to 471.36 ppm. LC50 of AuN ranged from 18.80 ppm to 41.52 ppm. In laboratory, the predatory efficiency of the cyclopoid crustacean Mesocyclops aspericornis against A. stephensi larvae was 26.8% (larva I) and 17% (larva II), while against A. aegypti was56% (I) and 35.1% (II). Predation against late-instar larvae was minimal. In AuN-contaminated environment,predation efficiency against A. stephensi was 45.6% (I) and 26.7% (II), while against A. aegypti was 77.3% (I) and 51.6% (II). Overall, low doses of AuN may help to boost the control of Anopheles and Aedes larval populations in copepod-based control programs.

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