Evaluation of adjuvant activity of fractions derived from Agaricus blazei, when in association with the recombinant LiHyp1 protein, to protect against visceral leishmaniasis

- Purification of polysaccharide-rich fractions from Agaricus blazei mushroom.
- In vitro immune stimulation experiments using spleen cells of BALB/c mice.
- Selection of the two best adjuvant fractions (F2 and F4).
- Protection of LiHyp1 protein plus F2, F4 or saponin against Leishmania infantum.
- Identification of new Th1 immune response adjuvants derived from Agaricus blazei.