Generation of stable L. major+EGFP-LUC and simultaneous comparison between EGFP and luciferase sensitivity

- Stable transgenic L. major expressing fused EGFP and firefly protein is feasible.
- Using two reporter genes simultaneously increases the experimental sensitivity.
- LUC was 10-fold more sensitive than EGFP in promastigote stage.
- EGFP and LUC are appropriate for in vitro and in vivo evaluations respectively.