Entamoeba histolytica antigenic protein detected in pus aspirates from patients with amoebic liver abscess

- 2-DE and Western blot were performed on three pools of ALA pus samples.
- Infected hamster sera and human amoebic-specific IgG were used as primary antibodies.
- A 14 kDa antigenic band was analysed by MALDI-TOF/TOF and LC-MS/MS.
- Tyrosine kinase was the most probable identification based on the spectrum analysis.