Identification and characterization of new major sperm protein genes from Oesophagostomum dentatum and Oesophagostomum quadrispinulatum from pigs in China

► New MSP genes with and without introns were identified from Oesophagostomum dentatum and Oesophagostomum quadrispinulatum. ► The MSP sequences without introns were 381 bp in length, coding 126 amino acids. ► MSP gene sequences containing introns were more conserved than those without introns. ► Amino acid sequence identities among the MSP sequences containing introns were 96.0-100%.