Detection of circulating antigen in serum of mice infected with Trichinella spiralis by an IgY-IgM mAb sandwich ELISA

► A novel ELISA to detect Trichinella spiralis circulating antigens were established. ► Chicken IgY used as a capture antibody and mouse IgM mAb as a detecting antibody. ► The assay could detect as little as 1 ng/ml of ES antigens. ► Circulating antigens were detected at 4 days post infection in sera of infected mice. ► This assay is valuable to the early diagnosis of trichinellosis.