Mice congenitally infected with low-to-moderate virulence Neospora caninum isolates exhibited clinical reactivation during the mating period without transmission to the next generation

- The present study started with a high number of congenitally infected mice.
- Low-to-moderate virulence N. caninum isolates were employed.
- A clinical reactivation of neosporosis was observed for the first time in mice.
- Nc-Spain 8 isolate was more efficient in inducing clinical reactivation.
- No EnTT was observed despite clinical reactivation in a previous generation.