Novel mutations in the antifolate drug resistance marker genes among Plasmodium vivax isolates exhibiting severe manifestations

► Novel mutations were observed in both DHFR and DHPS of Plasmodium vivax. ► Presence of novel mutations in PvDHPS gene suggests its polymorphic nature. ► Frequency of PvDHFR-PvDHPS two locus mutations were higher among the severe isolates.