A rapid method to assess the stage differentiation in Leishmania donovani by flow cytometry

► FCM is a non-flourimetric tool to study stage differentiation in Leishmania donovani. ► FCM was validated using different molecular and enzymatic assays. ► Partial CDS sequence (472 bp) of amastin gene was submitted in GenBank (HQ840734). ► Successful differentiation and propagation of LEM 138 were established.