Opisthorchis viverrini and Haplorchis taichui: Development of a multiplex PCR assay for their detection and differentiation using specific primers derived from HAT-RAPD

► Providing of 2 specific primers pairs. ► Can detect these 2 flukes in a single reaction multiplex PCR. ► Validates valuable diagnostic tool for identify at larval stages. ► Proposes a convenience method for construct specific primers.