Improvements in obtaining New World Leishmania sp from mucosal lesions: Notes on isolating and stocking parasites

► The ex vivo culture is adequate for the isolation of parasites from CL. ► The IFNγ-deficient mouse is recommended to isolate parasites from mucosal lesions. ► Stocking of Leishmania (Viannia) is critical to detect biological markers of these parasites.