Triplex PCR using new primers for the detection of Toxoplasma gondii

► We designed new primers from two Toxoplasma gondii gene targets. ► It detected T. gondii DNA in liver and spleen of mice infected with RH strain. ► It also detected 10 pg T. gondii DNA and 104 tachyzoites in spiked body fluids. ► It was 100% specific when tested with 10 other microorganisms and mouse muscle.