Occurrence and characterization of Acanthamoeba similar to genotypes T4, T5, and T2/T6 isolated from environmental sources in Brasília, Federal District, Brazil

► First occurrence of Acanthamoeba similar to genotypes T4, T5, and T2/T6, in Brasília, Brazil. ► Ten isolates grew at 37 °C and seven grew in media with 1.5 M mannitol. ► Identification of Acanthamoeba isolates from soil, swimming pools, and water features. ► Presence of potentially pathogenic Acanthamoeba reveals the risk of human infection.