Babesia bovis: A bipartite signal directs the glutamyl-tRNA synthetase to the apicoplast

► Protein traffic into the apicoplastic lumen requires a bipartite signal. ► Glutamyl tRNA synthetase (GltX) is a putative apicoplast targeted protein. ► GltX contains a bipartite signal sequence, a pre-requisite for apicoplast traffic. ► GltX bipartite sequence successfully directed GFP into the apicoplast. ► Native GltX may utilize its bipartite sequence for apicoplast entry.