Molecular cloning and characterization of leucine aminopeptidase from Fasciola gigantica

► The full length of cDNA encoding Fasciola gigantica LAP (FgLAP) was cloned from adult parasites. ► It was distributed in the apical cytoplasm of cecal epithelial cells, but not in both reproductive organs. ► The rFgLAP displayed the aminolytic activity by using fluorogenic substrate and inhibited by bestain.