Kinetics of parasite burdens in blood and tissues during murine toxoplasmosis

► Real-time PCR was used to monitor the distribution of Toxoplasma in murine tissues. ► We showed Toxoplasma appears in murine brains early after infection. ► Parasite burden reached in the lungs seems critical for survival. ► Persistent low-level parasitaemia was shown in infection with type-2 parasites. ► These are important baseline data for monitoring drug efficacy by real-time PCR.