Evaluation of DNA/DNA and prime-boost vaccination using LPG3 against Leishmania major infection in susceptible BALB/c mice and its antigenic properties in human leishmaniasis

LPG3 is the Leishmania homologue of the mammalian endoplasmic reticulum (ER) chaperone GRP94. This molecule has an essential role in synthesis of the lipophosphoglycan (LPG), the most abundant surface molecule identified on promastigotes of all Leishmania species. This paper describes the utilization of two vaccination strategies including DNA and prim/boost vaccination of LPG3 in susceptible model of Leishmania major infection. In addition different truncated forms of LPG3 were produce and sera reactivities of human CL and VL were tested as biomarker.