Identification of a 24Â kDa excretory secretory protein in Anisakis simplex

► A 24 kDa protein (22U homologous; As22U) was isolated from Anisakis simplex. ► As22U gene was isolated from A. simplex third-stage larvae cDNA library. ► As22U has signal peptide region and 16 highly conserved cysteine residues. ► As22U was identified in both the total extracts and ES proteins of A. simplex. ► The expression of TSLP, IL-25, and CXCL1 genes were increased by As22U treatment.