Complete sequence analysis of 18S rDNA based on genomic DNA extraction from individual Demodex mites (Acari: Demodicidae)

► Improving Demodex mite gDNA extraction and PCR examination technique. ► gDNA of single Demodex mite has been extracted successfully for the first time. ► It could satisfy the demands of Demodex 18S rDNA study at gene level. ► 18S rDNA is unsuitable for molecular identification at Demodex genus or below. ► 18S rDNA molecular data is suitable for family taxomomy in Cheyletoidea.