کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6292050 | 1302511 | 2010 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Protection against murine intestinal amoebiasis induced by oral immunization with the 29Â kDa antigen of Entamoeba histolytica and cholera toxin
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
انگل شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Protection against murine intestinal amoebiasis induced by oral immunization with the 29Â kDa antigen of Entamoeba histolytica and cholera toxin Protection against murine intestinal amoebiasis induced by oral immunization with the 29Â kDa antigen of Entamoeba histolytica and cholera toxin](/preview/png/6292050.png)
چکیده انگلیسی
Entamoeba histolytica antigens recognized by salivary IgA from infected patients include the 29Â kDa antigen (Eh29), an alkyl hydroperoxide reductase. Here, we investigate the potential of recombinant Eh29 and an Eh29-cholera toxin subunit B (CTxB) fusion protein to confer protection against intestinal amoebiasis after oral immunization. The purified Eh29-CTxB fusion retained the critical ability to bind ganglioside GM1, as determined by ELISA. Oral immunization of C3H/HeJ mice with Eh29 administered in combination with a subclinical dose of whole cholera toxin, but not as an Eh29-CTxB fusion, induced elevated levels of intestinal IgA and serum IgG anti-Eh29 antibodies that inhibited trophozoites adherence to MDCK cell monolayers. The 80% of immunized mice seen to develop IgA and IgG immune responses showed no evidence of infection in tissue sections harvested following intracecal challenge with virulent E. histolytica trophozoites. These results suggest that Eh29 is capable of inducing protective anti-amoebic immune responses in mice following oral immunization and could be used in the development of oral vaccines against amoebiasis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Parasitology - Volume 126, Issue 3, November 2010, Pages 359-365
Journal: Experimental Parasitology - Volume 126, Issue 3, November 2010, Pages 359-365
نویسندگان
J.C. Carrero, A. Contreras-Rojas, B. Sánchez-Hernández, P. Petrosyan, R.J. Bobes, L. Ortiz-Ortiz, J.P. Laclette,