Leishmania major: Protective capacity of DNA vaccine using amastin fused to HSV-1 VP22 and EGFP in BALB/c mice model

► Immunization with plasmid DNA encoding Leishmania antigens represents a promising approach for vaccination against leishmaniasis. ► DNA immunization with VP22-amastin-EGFP induces high response in terms of IFN-gamma production and protection against infectious challenge in mice. ► Co-administration of VP22 and EGFP could be an effective strategy to augment antigen immunogencity.