Concomitant and protective immunity in mice exposed to repeated infections with Echinostoma malayanum

Concomitant immunity and its consequence against infection play roles in regulating worm burdens in helminthiasis. Under natural conditions, this immunity is generated by exposure to repeated low dose or trickle infection. In this study, concomitant immunity was induced in mice exposed repeatedly to infection with Echinostoma malayanum and its protective effect on a challenge infection evaluated. A profile of worm burden from exposure to 10 metacercariae/mouse/week rose rapidly during the first 2 weeks reaching a plateau from week 3 to 8 post infection. Based on a cumulative dose of infection, worm recoveries were around 75% in the first 2 weeks, dropped to 50% at week 3 and 19% at week 8. After week 2, adult worm burden was constant and no juvenile worms were found after week 3 of the experiment. To examine the effect of resistance against reinfection, mice in the experimental group were primarily infected with 10 metacercariae/week for 5 weeks, treated with praziquantel and were challenged with 75 metacercariae/animal. The number of worms recovered from the experimental groups was significantly lower than that from naïve control groups beginning from 24 h to 28 days post challenge. The worms in the experimental group showed growth retardation and the proportion of adult worms was lower than that in the control animals especially during the first 3 weeks of the experiment. Parasite fecundity was also suppressed compared with that in the control group. The selective effects of protective immunity on establishment, growth, and fecundity of challenged worms affected the population dynamics of E. malayanum which is a similar phenomenon to concomitant immunity in schistosomiasis.

Worm recovery per animal of Echinostoma malayanum in mice exposed to weekly infections with 10 metacercariae/animal. Data shown are mean and SD (each data point calculated from a sample size of 5 animals) of juveniles (shaded bars), adults (open bars) and total worms (hatched bars).Research highlights► Mice immunized by repeated exposure to infection with Echinostoma malayanum developed concomitant immunity to re infection. ► Protective immunity reduces worm establishment has no impact on newly excysted juveniles (<24 h old). ► The worms in experimental groups showed growth retardation and the proportion of adult worms was significantly lower than that in the naïve control groups. ► Parasite fecundity was significantly suppressed compared with the naive controls.