کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6318757 | 1619193 | 2013 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Application of MicroResp⢠for soil ecotoxicology
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
شیمی زیست محیطی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Application of MicroResp⢠for soil ecotoxicology Application of MicroResp⢠for soil ecotoxicology](/preview/png/6318757.png)
چکیده انگلیسی
MicroResp⢠is a miniaturised method for measuring substrate induced respiration (SIR) in soil. We modified the MicroResp⢠method to develop a rapid tool for quantifying the ecotoxicological impact of contaminants. The method is based on reduction in SIR across a gradient of contaminant, allowing for determination of dose-response curves EC-values. Contaminants are mixed into soil samples at a range of concentrations; each sample is then dispensed into a column of eight wells in 96 well format (deep) plates. Moisture and glucose are added to the samples at levels to provide maximum response. Released CO2 from the soils is then measured using colorimetric gel-traps, following the standard MicroResp⢠methodology. Examination revealed that this method works over a range of soil types and is insensitive to minor variations in assay length (2-7 h), alteration of moisture content (±20 μL from optimum), and soil storage conditions (4 °C versus fresh).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Environmental Pollution - Volume 179, August 2013, Pages 177-184
Journal: Environmental Pollution - Volume 179, August 2013, Pages 177-184
نویسندگان
Steven Wakelin, Enzo Lombi, Erica Donner, Lynne MacDonald, Amanda Black, Maureen O'Callaghan,