Reproductive toxicity of bisphenol A and cadmium in Potamopyrgus antipodarum and modulation of bisphenol A effects by different test temperature

► We performed a reproduction test with the mollusc Potamopyrgus antipodarum. ► We defined the test substance concentration range for a pre-validation study. ► The bisphenol A effect (increased reproduction) depends on the test temperature. ► Reproduction of control groups significantly varies depending on temperature. ► The brood stock shows seasonal fluctuations in reproduction at constant conditions.