کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
633973 1456045 2014 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Fractionation of α-lactalbumin and β-lactoglobulin from bovine milk serum using staged, positively charged, tangential flow ultrafiltration membranes
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی تصفیه و جداسازی
پیش نمایش صفحه اول مقاله
Fractionation of α-lactalbumin and β-lactoglobulin from bovine milk serum using staged, positively charged, tangential flow ultrafiltration membranes
چکیده انگلیسی


• First to use charged UF membranes for fractionating proteins from milk serum.
• First to use natural feed solution at innate conductivity for this purpose.
• Proteins of chromatographic purity are made with no diafiltration, no water added.
• Fractionate proteins 15–20 times smaller than the membrane molecular weight cutoff.
• Mass balance models using single-stage data predict well multi-stage performance.

Fractionation of bovine α-lactalbumin (ALA) from β-lactoglobulin (BLG) in milk serum permeate (MSP) using tangential flow ultrafiltration was examined in this work. By placing a positive charge on a 300 kDa regenerated cellulose ultrafiltration membrane, it was possible to increase the selectivity for fractionating ALA and BLG by 180% compared to an uncharged membrane. Thus, like-sized proteins that differed only somewhat in isoelectric point, and that were about 15–20 times smaller than the membrane molecular weight cutoff, were fractionated using charged ultrafiltration membranes. Electrostatic repulsion was solely responsible for the improved selectivity. Protein sieving coefficients were explained by the net charge on the protein and the stagnant film model. From experimental data using a single-stage system under total recycle conditions, mass balance models were used successfully to predict the performance of a multi-stage membrane system. A three-stage system gave 87% pure ALA and 83% pure BLG. Thus, dairy protein fractions of chromatographic purity can be obtained using charged ultrafiltration membranes arranged in stages, without the wastewater generation and expense of chromatography. The results of this study will be useful for expanding the utility of ultrafiltration membranes beyond simple protein concentration to fractionation of proteins.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Membrane Science - Volume 454, 15 March 2014, Pages 488–495
نویسندگان
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