کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6377080 | 1624859 | 2013 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Expression analysis of key genes of phenylpropanoid pathway and phenol profiling during Ricinus communis-Fusarium oxysporum f. sp. ricini interaction
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موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم زراعت و اصلاح نباتات
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چکیده انگلیسی
The general phenylpropanoid metabolism generates an array of secondary metabolites and phenolic acids which imparts disease resistance in plants. Expression pattern of the key genes of phenylpropanoid pathway was studied at 0, 24 and 48Â h after infection (h.a.i.) and phenolic acid profiling was carried out at 0, 24, 48 and 72Â h.a.i. in the leaves of wilt infected and non- infected of both resistant and susceptible genotypes of castor. Expression analysis of PAL (phenylalanine ammonia lyase), C4H1 (cinnamate 4-hydroxylase 1) and C4H2 (cinnamate 4-hydroxylase 2) genes using RT-PCR with gene specific primers showed appreciable increase in the expression of PAL and C4H-2 gene in resistant genotypes at 48Â h interval than 24Â h interval compared to susceptible genotype. However, gene C4H-1 was down regulated in susceptible genotypes after 24 and 48Â h.a.i. while up regulated in resistant genotypes. Phenol profiling using HPTLC showed the presence of three phenolic acids i.e. caffeic acid, ferulic acid and salicylic acid in non-infected and infected castor genotypes. Higher content of caffeic and ferulic acid was detected in infected and non-infected resistant genotypes at 0, 24 and 48Â h.a.i, whereas caffeic acid was not detected in susceptible genotypes at 0Â h.a.i. These results suggest the critical role of phenols in castor disease resistance.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Industrial Crops and Products - Volume 50, October 2013, Pages 456-461
Journal: Industrial Crops and Products - Volume 50, October 2013, Pages 456-461
نویسندگان
Pritam R. Jadhav, Mahesh K. Mahatma, Lalit Mahatma, Sanjay Jha, Vipul B. Parekh, Vikas Khandelwal,