Cloning and functional analysis of the ecdysteroid receptor complex in the opossum shrimp Neomysis integer (Leach, 1814)

► We cloned the ecdysteroid receptor (EcR) and the retinoid-X-receptor (RXR) of the opposum shrimp Neomysis integer. ► We developed a chimeric Gal4 cell reporter system to investigate the heterodimerization of NiEcR-CrcRXR in vitro. ► PonA facilitated dimerization, while the insecticide tebufenozide with endocrine disruptive activities had no effect. ► In silico binding of tebufenozide with NiEcR revealed steric hindrance, while PonA fitted perfectly. ► Neomysis integer juveniles exposed to tebufenozide, in vivo, showed no molting disturbances.