Impacts of 17α-ethynylestradiol exposure on metabolite profiles of zebrafish (Danio rerio) liver cells

► We apply NMR-based metabolomics to study responses of ZFL cells exposed to EE2. ► The metabolomics approach has capability to capture cellular response to exposure. ► The analysis provides detailed molecular information on chemical's mode of action. ► Cellular metabolomics may have application for screening chemical exposure/toxicity.