The impact of associated bacteria on morphology and physiology of the dinoflagellate Alexandrium tamarense

- The influence of bacteria on growth and function of a dinoflagellate is characterized.
- Co-cultures of Alexandrium tamarense and bacteria were monitored for 15 months.
- Algal growth increased and photosynthesis improved in the presence of bacteria.
- Results do not support a role for algae-bacteria interactions in phycotoxin synthesis.
- New clues for the understanding of biosynthetic pathways of saxitoxin are presented.

Despite their potential impact on phytoplankton dynamics and biogeochemical cycles, biological associations between algae and bacteria are still poorly understood. The aim of the present work was to characterize the influence of bacteria on the growth and function of the dinoflagellate Alexandrium tamarense. Axenic microalgal cultures were inoculated with a microbial community and the resulting cultures were monitored over a 15-month period, in order to allow for the establishment of specific algal-bacterial associations. Algal cells maintained in these new mixed cultures first experienced a period of growth inhibition. After several months, algal growth and cell volume increased, and indicators of photosynthetic function also improved. Our results suggest that community assembly processes facilitated the development of mutualistic relationships between A. tamarense cells and bacteria. These interactions had beneficial effects on the alga that may be only partly explained by mixotrophy of A. tamarense cells. The potential role of organic exudates in the establishment of these algal-bacterial associations is discussed. The present results do not support a role for algal-bacterial interactions in dinoflagellate toxin synthesis. However, variations observed in the toxin profile of A. tamarense cells during culture experiments give new clues for the understanding of biosynthetic pathways of saxitoxin, a potent phycotoxin.