کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
638864 1456191 2006 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Immobilization of endo-1,4-β-xylanase on polysulfone acrylate membranes: Synthesis and characterization
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی تصفیه و جداسازی
پیش نمایش صفحه اول مقاله
Immobilization of endo-1,4-β-xylanase on polysulfone acrylate membranes: Synthesis and characterization
چکیده انگلیسی

We report on a new enzyme/support system to immobilize proteins such as enzymes through a covalent bond on polysulfone membranes. In the present case the enzyme endo-1,4-β-xylanase (E.C.3.2.1.8) is attached to polysulfone previously derivatized by introducing an acrylate group. Membranes are properly prepared from this polysulfone acrylate. Afterwards the enzyme is immobilized though the amino groups of side chains of the amino acids of the enzyme and the acrylate group of the derivatized polysulfone. Such immobilization of the enzyme is confirmed by microelemental analysis as well as by amino acid analysis by HPLC. Moreover, the enzymatic activity of the membranes was evaluated and compared with that corresponding to the free enzyme. Certain physical parameters (asymmetry, irregularity, pore size and surface roughness) of the corresponding enzymatic membranes were obtained from SEM and AFM image interpretation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Membrane Science - Volume 280, Issues 1–2, 1 September 2006, Pages 383–388
نویسندگان
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