Asymptomatic presence of Nosema spp. in Spanish commercial apiaries

► Honeybee samples (N = 604) were analysed using PCR with capillary electrophoresis. ► N. apis and N. ceranae are distributed throughout the Spanish territory. ► Both parasites are present in the hives without developing clinical signs of disease. ► There is no evidence for replacement of N. apis by N. ceranae.