Development and application of loop-mediated isothermal amplification assays on rapid detection of various types of staphylococci strains

► We established 3 LAMP detection methods for mecA, femA and 16S rRNA of Staphylococcus. ► The sensitivities of the LAMP assays were 10-1000 times higher than PCR. ► The LAMP assays were applied to 118 various Staphylococcus strains. ► The detection rates of 3 LAMP assays were 100%, 98.5% and 94.3%.