کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6398563 | 1330683 | 2012 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Characterization of a thermostable glucose isomerase with an acidic pH optimum from Acidothermus cellulolyticus
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش تغذیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Characterization of a thermostable glucose isomerase with an acidic pH optimum from Acidothermus cellulolyticus Characterization of a thermostable glucose isomerase with an acidic pH optimum from Acidothermus cellulolyticus](/preview/png/6398563.png)
چکیده انگلیسی
The glucose isomerase (GI) gene from the Acidothermus cellulolyticus 11B was cloned and overexpressed in Escherichia coli. The recombinant protein was purified to electrophoretical homogeneity by affinity chromatography using Chelating Sepharose Fast Flow resin. The enzyme activity was maximal at 80 °C and pH 6.5 while being significantly enhanced by Co2+ and Mg2+. The enzyme was determined as thermostable and weak-acid stable. These findings indicated that the GI from A. cellulolyticus 11B is appropriate for use as a new source of high-fructose corn syrup (HFCS) producing enzyme.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Research International - Volume 47, Issue 2, July 2012, Pages 364-367
Journal: Food Research International - Volume 47, Issue 2, July 2012, Pages 364-367
نویسندگان
Wanmeng Mu, Xiaole Wang, Qinghai Xue, Bo Jiang, Tao Zhang, Ming Miao,