Authentication of prized white and black truffles in processed products using quantitative real-time PCR

► Spores were identified through microscopic techniques in products containing truffles. ► A new method to extract DNA from food products was tested. ► New specific Tuber magnatum primers were developed. ► T. magnatum was authenticated and quantified in creams and butter by means of qPCR. ► T. melanosporum, which was not listed on the label, was detected.