کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6399389 | 1330696 | 2012 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Application of ergothioneine-rich extract from an edible mushroom Flammulina velutipes for melanosis prevention in shrimp, Penaeus monodon and Litopenaeus vannamei Application of ergothioneine-rich extract from an edible mushroom Flammulina velutipes for melanosis prevention in shrimp, Penaeus monodon and Litopenaeus vannamei](/preview/png/6399389.png)
This report investigated the melanosis-inhibiting properties of crude water soluble extract from the base and fruiting body waste of an edible mushroom (Flammulina velutipes) containing 9.13 ± 2.03 mg/mL 2-thiol-l-histidine-betaine (ergothioneine, ESH). Immersion of live full-grown black tiger shrimp (Penaeus monodon) and Pacific white shrimp (Litopenaeus vannamei) in a 0.5% w/v solution of mushroom extract for 1 h significantly decreased polyphenoloxidase (PPO) activity in shrimp hemolymph and expression of the prophenoloxidase (proPO) gene in hemocytes. Consequently, the development of melanosis in the treated shrimp during ice storage was prevented. Treatment with a 0.05% w/v solution of sodium sulfite and 4-hexyl-1,3-benzenediol had a similar effect. Enzyme assays showed that ESH is a non-competitive inhibitor. It is proposed that ESH possibly interacts directly with Cu2+ at the putative binding sites of PPO and proPO, based on copper-chelating activity analyses, thus preventing melanosis in the shrimp. This study indicated that application of ESH-rich F. velutipes mushroom extract from trimming waste is an effective natural alternative to synthetic melanosis-inhibiting agents to prevent postmortem melanosis in shrimp.
⺠Immersion of live shrimps in an EHS-rich mushroom extract inhibited melanosis. ⺠L-ESH inhibited PPO activity and suppressed proPO gene expression in shrimp hemocyte. ⺠L-ESH is a non-competitive inhibitor and has shown copper-chelating activity. ⺠Mushroom extract has same effect with sulfite and resorcinol on melanosis inhibition.
Journal: Food Research International - Volume 45, Issue 1, January 2012, Pages 232-237