کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6400207 | 1628522 | 2017 | 8 صفحه PDF | دانلود رایگان |
- The genotypic characterization revealed the presence of 12 distinct Enterococcus strains belonging to 5 different species in Turkish white cheese.
- Enterococcus faecium, E. faecalis, E. durans, E. gallinarum and E. italicus. presented in white cheese.
- Two strains showed resistance to vancomycin and cylB gene related to cytolysin metabolism presented in another two Enterococcus strains.
- All strains showed antimicrobial effects against food-borne pathogens with presence of genes related to enterocin production.
Enterococcus species was isolated from Turkish white cheese and genotypic characterization of these isolates revealed the presence of 12 distinct strains belonging to 5 different species: Enterococcus faecium, E. faecalis, E. durans, E. gallinarum and E. italicus. All strains showed good survival ability under bile salt conditions. The antibiotic resistance of these strains was low but two strains showed high levels of resistance including resistance to vancomycin. PCR detection of virulence determinant in these isolates revealed that cheese isolate enterococci contained virulence genes common in gut and food originated enterococci and importantly two strains harboured cylB gene related to cytolysin metabolism. No complete hemolytic activity was observed for Enterococcus strains but partial hemolytic activity was observed 8 out of 12 strains. Additionally, all strains showed important levels of antimicrobial activity against food-borne pathogens and PCR screening of genes encoding enterocin A and B indicated the presence of enterocin B gene in all tested strains. The antimicrobial activities of the tested strains were lost after proteolytic enzyme treatments but no alteration was observed at different pHs and after heat treatments. In summary, this study reflected characteristics of Enterococcus strains presented in Turkish white cheese in terms of functional and safety perspectives.
Journal: LWT - Food Science and Technology - Volume 75, January 2017, Pages 358-365