Chondroitin sulfate extraction from broiler chicken cartilage by tissue autolysis

► An autolysis method was used to extract chondroitin sulfate from chicken cartilage. ► Most (>90%) of uronic acid was extracted from the cartilage tissues. ► Chondroitin sulfate chain had 4-sulfate disaccharide as a predominant disaccharide. ► Chondroitin sulfate-peptide contained serine as a predominant amino acid.