کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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640572 | 1456970 | 2015 | 8 صفحه PDF | دانلود رایگان |

• Polyols-based deep eutectic solvents (PDES) are green extraction solvents.
• PDES-MAE was an efficient and green sample preparation method.
• It was applied for the extraction and determination of phenolic compounds from pyrola.
• A sensitive determination method was developed for analysis of 5 compounds in pyrola.
A group of green solvent named polyols-based deep eutectic solvents (PDES) combined with microwave assisted extraction (MAE) was first successfully applied in extracting phenolic compounds from pyrola. A series of PDES with mixing different ratios choline chloride (ChCl) to different polyols were investigated for extraction, and ChCl/1,4-Butanediol (1/4) was found to be the suitable extraction solvent. MAE was more effective than heat-stirring extraction and ultrasonic assisted extraction with PDES. The extraction parameters were optimized by BBD test combined with response surface methodology. The optimal extraction conditions were 30% of water in ChCl/1,4-Butanediol (1/4) at 70 °C for 20 min and liquid/solid ratio 10 mL/g. Moreover, RP-HPLC-UV was developed for the determination of five main active components in pyrola. This method exhibited satisfactory linearity (R2 > 0.999), precision (RSD < 4.03%) and recovery (96.17–103.25%). Sample analysis showed that the contents of hyperin, 2′-O-galloylhyperin, quercitrin, quercetin-O-rhamnoside and chimaphilin in pyrola were 1.627, 4.958, 0.041, 0.089 and 0.349 mg/g, respectively. These results revealed that PDES would be promising green solvents for analytical sample preparation from plant herbs.
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Journal: Separation and Purification Technology - Volume 149, 27 July 2015, Pages 116–123