Effects of ingredient composition on optical and mechanical properties of pullulan film for food-packaging applications

In the present research, the effects of pullulan (Pul), glycerin (Gly), xanthan gum (Xa) and locust bean (Lb) concentrations on pullulan film properties were investigated using a Central Composite Rotatable Design. Optimal ingredient combination was determined and antimicrobial activity of films combined with sakacin A was confirmed against Listeria monocytogenes. Using predictive models, contour plots and the characteristics of commercial LDPE films as constraints, the following combination within the optimal region was selected: Pul 100 g/l, Gly 10 g/l, Xa 1 g/l and Lb 1 g/l. Statistical analysis demonstrated that Pul and Gly significantly influenced the properties of pullulan films. Strong interactions were observed between Pul-Gly and Xa-Gly. When sakacin A was added to the film mixture, no significant influence on films optical properties was reported, while an increase in flexibility was observed. Results obtained indicate the potential application of pullulan film developed in this study as an effective antimicrobial biopolymer in food-packaging systems.

► We evalauted the effect of four ingredients concentration on pullulan film properties. ► We used a Central Composite Rotatable Design to determine optimal combinations. ► Pullulan and Glycerin significantly influenced films properties. ► When the bacteriocin sakacin A was added to film formulation no significant changes were reported in film formulation. ► Results showed application of pullulan film as antimicrobial active packaging system.