کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6406698 1628801 2015 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Short communicationIdentification of appropriate reference genes for RT-qPCR analysis in Ziziphus jujuba Mill.
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش باغداری
پیش نمایش صفحه اول مقاله
Short communicationIdentification of appropriate reference genes for RT-qPCR analysis in Ziziphus jujuba Mill.
چکیده انگلیسی


- This is the first report regarding the selection of appropriate reference genes for RT-qPCR analysis in jujube (Ziziphus jujuba Mill.).
- The most stable set of reference genes were screened for fruit developmental stages, tissues and genotypes of jujube.
- None of the reference genes were consistently expressed across all experimental samples evaluated.

Reverse transcription quantitative real-time PCR (RT-qPCR) is the most widely used method to evaluate the expression levels of mRNA, and the stability of reference genes is crucial for correct evaluation of RT-qPCR data. In the present work, 11 candidate reference genes were investigated for their expression levels at six fruit developmental stages and among four tissues and five genotypes of Ziziphus jujuba Mill. The geNorm, Normfinder and Bestkeeper software programs were applied to estimate the stability of the 11 reference genes. The results showed that UBQ, ACTIN, CYP and ACTIN9 for the fruit developmental stages, UBQ2 and UBQ for the different tissues, UBQ2, GAPDH, ACTIN9 and EF1 for the different genotypes, and UBQ, ACTIN9, UBQ2 and CYP for all samples evaluated were stably expressed. Additionally, the relative GME expression level was used to validate the reference genes. The findings may enable more accurate assessments of gene expression in Z. jujuba Mill.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Scientia Horticulturae - Volume 197, 14 December 2015, Pages 166-169
نویسندگان
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