کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
641391 | 1456996 | 2014 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Synchronous extraction of lignin peroxidase and manganese peroxidase from Phanerochaete chrysosporium fermentation broth
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
تصفیه و جداسازی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Synchronous extraction of lignin peroxidase and manganese peroxidase from Phanerochaete chrysosporium fermentation broth Synchronous extraction of lignin peroxidase and manganese peroxidase from Phanerochaete chrysosporium fermentation broth](/preview/png/641391.png)
چکیده انگلیسی
Two extracellular peroxidases from Phanerochaete chrysosporium, named lignin peroxidase (LiP) and manganese peroxidase (MnP), were extracted simultaneously by biosurfactant-based reversed micellar extraction method. Rhamnolipid (RL) was applied during the experiment. N-hexanol was chosen for the extraction from five different co-solvents including n-butanol, n-pentanol, n-hexanol, n-heptanol, and n-octanol. Parameters (RL concentration, salt concentration, pH, temperature, extraction time, and ethanol dosage) in forward and backward extraction process were investigated. Under the optimum conditions, the highest activity recoveries of LiP and MnP were up to 93.56% and 88.79%, respectively. This observation may be helpful in developing a more effective method to monitor the process of synchronous extraction of different enzymes by reversed micellar extraction.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Separation and Purification Technology - Volume 123, 26 February 2014, Pages 164-170
Journal: Separation and Purification Technology - Volume 123, 26 February 2014, Pages 164-170
نویسندگان
Xin Peng, Xing-zhong Yuan, Guang-ming Zeng, Hua-jun Huang, Hou Wang, Huan Liu, Shan Bao, Yu-jie Ma, Kai-long Cui, Li-jian Leng, Zhi-hua Xiao,