Influence of peptides and proteins produced by cyanobacterium Microcystis aeruginosa on the coagulation of turbid waters

• Peptides/proteins of Microcystis aeruginosa contribute to the coagulation of kaolin.
• Mechanisms of interactions between coagulating constituents are pH dependent.
• The optimum pH for kaolin and peptide/protein removal by Al/Fe is 4–6.5.
• Peptides/proteins form aggregates with kaolin even in the absence of coagulant.

The study investigated the influence of cellular peptides and proteins derived from cyanobacterium Microcystis aeruginosa on the coagulation of kaolin particles during water treatment. To describe the coagulation mechanisms, coagulation system constituents (peptides/proteins, kaolin and coagulant) were characterized in terms of their surface charges. The removal mechanisms of peptides/proteins and kaolin were evaluated by the comparison of the coagulation tests performed with and without coagulant (ferric or aluminum sulfate). We confirmed the peptide/protein inhibiting effect on coagulation through the formation of dissolved complexes with coagulants at a pH value of about 6 for Fe and a pH value of about 6.8 for Al. On the other hand, we demonstrated that cyanobacterial peptides/proteins also have positive effects as they induce the coagulation of hydrophobic kaolin particles within the pH range 4–6 for Fe and 5–6.5 for Al. Interestingly, when peptides/proteins bear a sufficiently low amount of negative charge (pH < 4.5), they can coagulate with kaolin by means of electrostatic interactions even in the absence of a coagulant. The study showed that peptides/proteins produced by M. aeruginosa can serve as coagulation aids and contribute to the turbidity removal at pH values below neutral (pH < 6 for Fe and pH < 6.5 for Al).

Figure optionsDownload as PowerPoint slide